human tumor brain cell line u87  (ATCC)

Journal: International Journal of Molecular Sciences

Article Title: Anti-Proliferative Potential of Cynaroside and Orientin—In Silico (DYRK2) and In Vitro (U87 and Caco-2) Studies

doi: 10.3390/ijms242316555

Figure Lengend Snippet: Anti-proliferative potential of 7-Lut and 8-Lut tested on human tumor glial cell line U87, by MTS assay. ( A , B ) cell viability after 24 h of exposure to 7-Lut and 8-Lut. ( C , D ) cell viability after 48 h of exposure to 7-Lut and 8-Lut. ( n = 3). Notation (*) means results without statistical significance ( p > 0.05); (**) means results with statistical significance (0.05 < p < 0.01); (***) means results with high statistical significance ( p < 0.01); 7-Lut means luteolin-7- O -glucoside; 8-Lut means luteolin-8- C -glucoside; MTS means 3-(4,5-dimethyl-thiazol-2-yl)-5-(3-carboxy-methoxy- phenyl)-2 -(4-sulfophenyl)-2H-tetrazolium (MTS).

Article Snippet: The cell lines investigated were the human tumor brain cell line U87 MG(ATCC-HTB-14) and the human colorectal carcinoma Caco-2 cells (ATCC, HTB-37).

Techniques: MTS Assay

Journal: International Journal of Molecular Sciences

Article Title: Anti-Proliferative Potential of Cynaroside and Orientin—In Silico (DYRK2) and In Vitro (U87 and Caco-2) Studies

doi: 10.3390/ijms242316555

Figure Lengend Snippet: The viability response of U87 cells to 7-Lut and 8-Lut after 24 and 48 h of exposure.

Article Snippet: The cell lines investigated were the human tumor brain cell line U87 MG(ATCC-HTB-14) and the human colorectal carcinoma Caco-2 cells (ATCC, HTB-37).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Potential Benefits of Dietary Plant Compounds on Normal and Tumor Brain Cells in Humans: In Silico and In Vitro Approaches

doi: 10.3390/ijms24087404

Figure Lengend Snippet: Effect of test reference compounds (caffeic acid, gentisic acid, ferulic acid and para-aminobenzoic acid/PABA) on human normal brain cell line NHA and human tumor brain cell line U87, in cytotoxicity MTS assay. ( A ) NHA cell line viability after 24 h of exposure to test reference compounds. ( B ) U87 cell line viability after 24 h of exposure to test reference compounds. Data were the mean of three replicates (n = 3). Note: * = results without statistical significance ( p > 0.05); ** = results with statistical significance ( p < 0.05).

Article Snippet: Accordingly, MTS tests were performed on human normal brain cell line NHA (CC-2565, Lonza, Basel, Switzerland) and human tumor brain cell line U87 MG (ATCC-HTB-14, Tell City, IN, USA).

Techniques: MTS Assay

Journal: International Journal of Molecular Sciences

Article Title: Potential Benefits of Dietary Plant Compounds on Normal and Tumor Brain Cells in Humans: In Silico and In Vitro Approaches

doi: 10.3390/ijms24087404

Figure Lengend Snippet: Effect of test reference compounds (caffeic acid, gentisic acid, ferulic acid and para-aminobenzoic acid/PABA) on human tumor brain cell line U87, in anti-proliferative MTS assay. ( A ) U87 cell line viability after 24 h of exposure to test reference compounds. ( B ) U87 cell line viability after 48 h of exposure to test reference compounds. Data were the mean of three replicates (n = 3). Note: * = results without statistical significance ( p > 0.05); ** = results with statistical significance ( p < 0.05).

Article Snippet: Accordingly, MTS tests were performed on human normal brain cell line NHA (CC-2565, Lonza, Basel, Switzerland) and human tumor brain cell line U87 MG (ATCC-HTB-14, Tell City, IN, USA).

Techniques: MTS Assay

Journal: International Journal of Molecular Sciences

Article Title: Potential Benefits of Dietary Plant Compounds on Normal and Tumor Brain Cells in Humans: In Silico and In Vitro Approaches

doi: 10.3390/ijms24087404

Figure Lengend Snippet: Effect of crude ethanolic extract from fresh Anemone nemorosa L. plant material (AN1) on human normal brain cell line NHA and human tumor brain cell line U87, in cytotoxicity MTS assay. ( A ) NHA cell line viability after 24 h of exposure to test vegetal extract AN1. ( B ) U87 cell line viability after 24 h of exposure to test vegetal extract AN1. All determinations were made in triplicate series, and the results are calculated as an average. Graphs A and B also present the percentage of cell viability by comparison to negative control series (CN). According to the statistical calculation, the results of the NHA and U87 cell lines in the cytotoxicity experiment were not statistically significant ( p > 0.05, n = 3).

Article Snippet: Accordingly, MTS tests were performed on human normal brain cell line NHA (CC-2565, Lonza, Basel, Switzerland) and human tumor brain cell line U87 MG (ATCC-HTB-14, Tell City, IN, USA).

Techniques: MTS Assay, Comparison, Negative Control

Journal: International Journal of Molecular Sciences

Article Title: Potential Benefits of Dietary Plant Compounds on Normal and Tumor Brain Cells in Humans: In Silico and In Vitro Approaches

doi: 10.3390/ijms24087404

Figure Lengend Snippet: Effect of crude ethanolic extract from fresh Anemone nemorosa L. plant material (AN1) on human tumor brain cell line U87, in anti-proliferative MTS assay. ( A ) U87 cell line viability after 24 h of exposure to test vegetal extract AN1; ( B ) U87 cell line viability after 48 h of exposure to test vegetal extract AN1. All determinations were made in triplicate series, and the results are calculated as an average. The graphs present the percentage of the cell viability in series by comparison to positive control sample (CP) series. According to statistical calculation, the results on the U87 cells at 24 h and 48 h were not statistically significant ( p > 0.05).

Article Snippet: Accordingly, MTS tests were performed on human normal brain cell line NHA (CC-2565, Lonza, Basel, Switzerland) and human tumor brain cell line U87 MG (ATCC-HTB-14, Tell City, IN, USA).

Techniques: MTS Assay, Comparison, Positive Control

Journal: International Journal of Molecular Sciences

Article Title: Potential Benefits of Dietary Plant Compounds on Normal and Tumor Brain Cells in Humans: In Silico and In Vitro Approaches

doi: 10.3390/ijms24087404

Figure Lengend Snippet: Effect of hot ethanolic extract from dried Anemone nemorosa L. plant material (AN2) on human normal brain cell line NHA and human tumor brain cell line U87, in cytotoxicity MTS assay. ( A ) NHA cell line viability after 24 h of exposure to test vegetal extract AN2. ( B ) U87 cell line viability after 24 h of exposure to test vegetal extract AN2. All determinations were made in triplicate series, and the results are calculated as an average. The graphs present the percentage of cell viability in each dilution point series by comparison to negative control point series (CN). By statistical calculation, the results on the NHA cell line were not statistically significant ( p > 0.05, n = 3), while the results on the U87 cell lines were statistically significant ( p < 0.05, n = 3).

Article Snippet: Accordingly, MTS tests were performed on human normal brain cell line NHA (CC-2565, Lonza, Basel, Switzerland) and human tumor brain cell line U87 MG (ATCC-HTB-14, Tell City, IN, USA).

Techniques: MTS Assay, Comparison, Negative Control

Journal: International Journal of Molecular Sciences

Article Title: Potential Benefits of Dietary Plant Compounds on Normal and Tumor Brain Cells in Humans: In Silico and In Vitro Approaches

doi: 10.3390/ijms24087404

Figure Lengend Snippet: Effect of hot ethanolic extract from dried Anemone nemorosa L. plant material (AN2) on human tumor brain cell line U87, in anti-proliferative MTS assay. ( A ) U87 cell line viability after 24 h of exposure to test vegetal extract AN2. ( B ) U87 cell line viability after 48 h exposure to test vegetal extract AN2. All determinations were made in triplicate series, and the results are calculated as an average. The graphs present the percentage of cell viability in each dilution point series by comparison to negative control point series (CN). By statistical calculation, the results on the U87 after 24 h of exposure to AN2 are statistically significant ( p < 0.05, n = 3), while the results at 48 h were not statistically significant ( p > 0.05, n = 3).

Article Snippet: Accordingly, MTS tests were performed on human normal brain cell line NHA (CC-2565, Lonza, Basel, Switzerland) and human tumor brain cell line U87 MG (ATCC-HTB-14, Tell City, IN, USA).

Techniques: MTS Assay, Comparison, Negative Control

Journal: Cancer science

Article Title: Inhibition of ADAM17 reduces hypoxia-induced brain tumor cell invasiveness.

doi: 10.1111/j.1349-7006.2007.00440.x

Figure Lengend Snippet: Fig. 2. Effect of the a disintegrin and metalloproteinase (ADAM) 17 inhibitor tumor necrosis factor-α protease inhibitor (TAPI)-2 on the in vitro invasiveness of U87 brain tumor cells cultured under normoxic (20% O2) or hypoxic (1% O2) conditions. (a) Representative pictures of U87 cell invasiveness assay with different treatments. 1, Normoxic control; 2, normoxia + 20 µmol/L TAPI-2; 3, hypoxia; 4, hypoxia + 20 µmol/ L TAPI-2. (b) Relative invasive cell number compared to normoxic control. Data are shown as percentage of results under normoxia. The values indicated by two asterisks (**) were significantly different (P < 0.01) from normoxia; the values indicated by hatches ( #P < 0.01) were significantly different from hypoxic control.

Article Snippet: The 9L rat brain tumor and U87 human brain tumor cell lines were obtained from American Type Culture Collection (ATCC, Rockville, MD, USA).

Techniques: Protease Inhibitor, In Vitro, Cell Culture, Control

Journal: Cancer science

Article Title: Inhibition of ADAM17 reduces hypoxia-induced brain tumor cell invasiveness.

doi: 10.1111/j.1349-7006.2007.00440.x

Figure Lengend Snippet: Fig. 7. Enzyme-linked immunosorbent assay analysis for phospho-extracellular signal-regulated kinase (p-ERK) and phospho-serine/threonine kinase (p-AKT) protein levels in 9L and U87 cells. 9L and U87 cells were cultured under normoxic and hypoxic conditions for 24 h. p-AKT levels in both cell lines were significantly increased under hypoxic conditions, compared to normoxic conditions. Treatment with tumor necrosis factor- α protease inhibitor (TAPI)-2 (20 µmol/L) decreased p-AKT level significantly in both (b) 9L (28.2% inhibition compared to hypoxia control) and (d) U87 (37.4% inhibition compared to hypoxia control) cell lines. However, hypoxia and treatment of TAPI-2 did not alter the levels of p- ERK2 in either (a) 9L or (c) U87 cell lines.

Article Snippet: The 9L rat brain tumor and U87 human brain tumor cell lines were obtained from American Type Culture Collection (ATCC, Rockville, MD, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Cell Culture, Protease Inhibitor, Inhibition, Control

Journal: Cancer science

Article Title: Inhibition of ADAM17 reduces hypoxia-induced brain tumor cell invasiveness.

doi: 10.1111/j.1349-7006.2007.00440.x

Figure Lengend Snippet: Fig. 9. Effect of human a disintegrin and metalloproteinase (ADAM) 17 small interfering RNA (siRNA) transfection on the in vitro invasiveness of U87 brain tumor cells cultured under normoxic (20% O2) or hypoxic (1% O2) conditions. (a) Representative pictures of U87 cell invasion assay with different treatments. 1, Cells under normoxic conditions transfected with control siRNA; 2, cells under normoxic conditions transfected with ADAM17 siRNA; 3, cells under hypoxic conditions transfected with control siRNA; 4, cells under hypoxic conditions transfected with ADAM17 siRNA. (b) Relative invasive cell number compared to normoxic control. Data are shown as percentage of results under normoxic conditions. The values indicated by two asterisks (**) were significantly different (P < 0.01) from normoxia; the values indicated by hatches (#P < 0.01) were significantly different from the hypoxic control.

Article Snippet: The 9L rat brain tumor and U87 human brain tumor cell lines were obtained from American Type Culture Collection (ATCC, Rockville, MD, USA).

Techniques: Small Interfering RNA, Transfection, In Vitro, Cell Culture, Invasion Assay, Control

Journal: Cancer science

Article Title: Inhibition of ADAM17 reduces hypoxia-induced brain tumor cell invasiveness.

doi: 10.1111/j.1349-7006.2007.00440.x

Figure Lengend Snippet: Fig. 10. Western blot analysis for a disintegrin and metalloproteinase (ADAM) 17, epidermal growth factor receptor (EGFR), phosphorylated EGFR (p-EGFR), serine/threonine kinase (AKT) and phosphorylated AKT (p-AKT) protein levels in U87 cells after small interfering RNA (siRNA) transfection. U87 cells transfected with siRNA cultured under normoxic and hypoxic conditions for 24 h. Protein levels of ADAM17, p-EGFR, and p-AKT were all significantly increased under hypoxic conditions; protein levels of EGFR and AKT were not significantly altered by hypoxia. ADAM17 siRNA transfection decreased ADAM17, p-EGFR and p-AKT protein levels in both normoxic and hypoxic conditions. EGFR and AKT protein levels were not significantly altered by ADAM17 siRNA in either normoxic or hypoxic conditions.

Article Snippet: The 9L rat brain tumor and U87 human brain tumor cell lines were obtained from American Type Culture Collection (ATCC, Rockville, MD, USA).

Techniques: Western Blot, Small Interfering RNA, Transfection, Cell Culture

Journal: Cancer science

Article Title: Inhibition of ADAM17 reduces hypoxia-induced brain tumor cell invasiveness.

doi: 10.1111/j.1349-7006.2007.00440.x

Figure Lengend Snippet: Fig. 11. Effects of AG1478 and LY294002 on the epidermal growth factor receptor (EGFR), phosphorylated EGFR (p-EGFR), serine/threonine kinase (AKT) and phosphorylated AKT (p-AKT) protein levels in U87 cells under normoxic and hypoxic conditions. U87 cells were treated with EGFR activation inhibitor AG1478 and AKT activation inhibitor LY294002 under normoxic and hypoxic conditions. Protein levels of p-EGFR and p- AKT were all significantly increased under hypoxic conditions; protein levels of EGFR and AKT were not significantly altered by hypoxic treatment. AG1478 decreased p-EGFR and p-AKT protein levels in both normoxic and hypoxic conditions; EGFR and AKT protein levels were not significantly altered by AG1478 in either normoxic or hypoxic conditions. LY294002 decreased p-AKT protein levels in both normoxic and hypoxic conditions; p-EGFR, EGFR and AKT protein levels were not significantly altered by LY294002 in either normoxic or hypoxic conditions.

Article Snippet: The 9L rat brain tumor and U87 human brain tumor cell lines were obtained from American Type Culture Collection (ATCC, Rockville, MD, USA).

Techniques: Activation Assay